Journal: BMC Oral Health

Article Title: Comparative evaluation of color stability in polished and glazed CAD/CAM ceramics subjected to coffee thermal cycling: an in vitro study

doi: 10.1186/s12903-025-05905-7

Figure Lengend Snippet: Ceramic composition

Article Snippet: Suprinity LS , Lithium Silicate Zirconia-Reinforced Ceramic , SiO2 (56–64) Li2O (15–21) ZrO2 (8–12) P2O5 (3–8) K2O (1–4) Al2O3 (1–4) , Vita Zahnfabrik, Germany.

Techniques:

Journal: Journal of Virology

Article Title: Inherited chromosomally integrated human herpesvirus 6: regional variation in prevalence, association with angina, and identification of ancestral viral lineages in two large UK studies

doi: 10.1128/jvi.02160-24

Figure Lengend Snippet: HHV-6 integrations and ancestral viral lineages. (a) Manhattan plot showing an association between iciHHV-6B and imputed genetic variants close to the telomeres of chromosome 7p, 9q, 11p, 17p, and 19q. (b) Analysis of samples with clade B6 iciHHV-6B genomes by optical genome mapping. Consensus maps (cmaps, green bars) from optical genome mapping were assembled using the human genome (hg38 build, yellow bar) as a reference on the basis of fluorescent labeling of CTTAAG recognition sites that aligned to the human reference (blue vertical lines) or did not align to the human reference (red vertical lines). The pattern of fluorescent labels unique to the HHV-6B genome (gray bar) could be identified in unaligned fluorescent labels at the ends of cmaps that aligned to chromosome 7 p, 9q, and 21q for GLA_21713, GLA_32597, and GLA_13910, respectively. (c) Patterns of telomere and telomere-like repeats in the junction fragment between iciHHV-6 and low copy-number sequence in the subtelomere. Each small rectangle represents a telomere or telomere-like sequence as indicated in the Repeat key. * Denotes an omitted telomere-repeat array of 290 hexamers, 65% TTAGGG interspersed with telomere-likes repeat, almost entirely TTAGAC-CTAGGG dimers. B6_9q_1 refers to B6_9q_hap1 (B6_9q_T2), B6_9q_2 refers to B6_9q_hap2 (B6_9q_T1). (d) Evolutionary analysis by Maximum Likelihood method of 20 iciHHV-6B genome sequences from GS:SFHS and seven publicly available iciHHV-6B genome sequences. Branch tips are labeled with sample name and lineage, where known. The evolutionary history was inferred using the Maximum Likelihood method and Tamura-Nei model . Branch lengths are measured in the number of substitutions per site. There were a total of 140,246 positions in the final data set.

Article Snippet: Thermal cycling was performed on a Bio-Rad Laboratories C1000 Touch Thermo Cycler (94°C for 2 min; 31 or 40 cycles of 94°C for 15 s, 64°C for 30 s, 68°C for 2 min; 68°C 7 min [17p] or 2 min [9q]).

Techniques: Labeling, Low Copy Number, Sequencing